The following methods can be used to deal with L-proline after it has been affected by moisture:
I. Drying Treatment
Low-temperature Drying: If the degree of moisture absorption of L-proline is relatively slight, it can be placed in an oven and dried at a low temperature. Generally, the temperature should be controlled at around 40 - 60°C to avoid the decomposition or deterioration of L-proline caused by excessively high temperatures. During the drying process, it is advisable to stir the material appropriately to ensure uniform heating and accelerate the evaporation of water.
Vacuum Drying: For L-proline that has high requirements for humidity and whose quality is easily affected after absorbing moisture, vacuum drying is a preferable method. In a vacuum environment, the boiling point of water decreases, and the water can be quickly removed at a lower temperature, reducing the degradation and oxidation of L-proline during the drying process. Put the moisture-affected L-proline into a vacuum drying oven, set appropriate temperature (such as 50 - 70°C) and vacuum degree (usually 10 - 100Pa), and dry it until the water content meets the requirements.
Using Desiccants: The moisture-affected L-proline can be placed in a closed container together with desiccants. The desiccants can absorb the surrounding moisture, thus gradually drying the L-proline. Commonly used desiccants include silica gel, anhydrous calcium chloride, etc. Put the desiccants in a breathable bag, place them in a sealed container together with L-proline. After a period of time, the desiccants will absorb the moisture in the container, achieving the purpose of drying the L-proline.
II. Screening and Crushing (if necessary)
Screening: If the moisture-affected L-proline has formed lumps, the larger lumps can be separated first by screening. Use a sieve with an appropriate mesh number to sieve out the non-lumped L-proline, and the lumped part can be further processed.
Crushing: For the harder lumped part, the crushing method can be used to break it into smaller particles for better drying treatment. Tools such as a mortar and pestle or a crusher can be used for crushing, but care should be taken to avoid introducing impurities during the crushing process.
III. Quality Inspection
The treated L-proline needs to undergo quality inspection, including the detection of indicators such as water content, purity, and impurity content. The Karl Fischer method can be used to determine the water content, and methods such as high-performance liquid chromatography can be used to detect the purity and impurity content. Only when all indicators meet the relevant standards and requirements can this L-proline be used or sold continuously.
When dealing with the moisture-affected L-proline, attention should be paid to the hygiene and safety during the operation process to prevent it from being contaminated or having other quality problems. At the same time, the moisture-affected L-proline should be dealt with as soon as possible to reduce the adverse impact on its quality caused by prolonged moisture absorption.